Genome-greater single nucleotide polymorphism (SNP) indicators along with allele amount recommendations keeps emerged since an effective tool to own studying state-of-the-art characteristics during the grown autotetraploid potato (Solanum tuberosum L., 2n = 4? = 48). At this point, this method might have been efficiently put on the fresh character off quantitative trait loci (QTLs) fundamental highly heritable qualities like condition opposition, however, mainly unexplored to possess faculties which have cutting-edge activities away from genetics.
Abilities
In this study, an Fstep 1 tetraploid russet mapping population (162 individuals) was evaluated for multiple quantitative traits over two years and two locations to identify QTLs associated with tuber sugar concentration, processing quality, vine maturity, and other high-value agronomic traits. We report the linkage maps for the 12 potato chromosomes and the QTL location with corresponding genetic models and candidate SNPs explaining the highest phenotypic variation for tuber quality and maturity related traits. Significant QTLs for tuber glucose concentration and tuber fry color were detected on chromosomes 4, 5, 6, 10, and 11. Collectively, these QTLs explained between 24 and 46% of the total phenotypic variation for tuber glucose and fry color, respectively. The QTL on chromosome 10 was associated with apoplastic invertases, with ‘Premier Russet’ contributing the favorable allele for fry processing quality. On chromosome 5, minor-effect QTLs for tuber glucose concentration escort Cleveland OH and fry color co-localized with various major-effect QTLs, including vine maturity, growth habit, tuber shape, early blight (Altenaria tenuis), and Verticillium wilt (Verticillium spp.).
Conclusions
Linkage data and QTL mapping from inside the a russet mapping inhabitants (A05141) having fun with SNP dosage guidance successfully recognized positive alleles and applicant SNPs to have effectiveness the brand new buildup from tuber cutting sugars. These novel markers have a leading prospect of the advance out-of tuber handling quality. Additionally, the latest breakthrough of different hereditary models having faculties with overlapping QTLs at the maturity locus demonstrably indicates an independent hereditary control.
Records
That have an elaborate polyploid family genes brand new grown autotetraploid potato (Solanum tuberosum L., 2n = 4? = 48) merchandise novel demands having linkage mapping and you may quantitative trait loci (QTL) research. New current growth of potato solitary nucleotide polymorphism (SNP) arrays provides a collection of genome-greater indicators that can be used in the mapping and you will quantitative trait locus (QTL) data [step 1,dos,3,4]bined to your method of getting statistical gadgets that enable the utilization out of allele dosage information (we.e., what amount of copies each and every allele from the a given polymorphic locus), it is currently you’ll be able to in order to rather enhance the energy regarding SNP pointers so you can detect recombination occurrences and also to make use of this information toward QTL mapping [5,6,7]. Up until now, this process might have been efficiently familiar with discover novel QTLs and you can applicant SNPs from the late blight opposition [4, 8], interior temperature necrosis , Potato virus Y , and you can agronomic traits , however, mostly unexplored to possess reading handling quality or other higher-really worth qualities that have complex inheritance.
There is an importance of molecular indicators regarding tuber control top quality faculties to greatly help your choice of germplasm to own cultivar creativity. Handling quality is simply determined by tuber sugar blogs. The newest buildup away from fructose and you will sugar (reducing sugars) within the potato tubers throughout the cold storage negatively affects the finish-use top quality properties. Cutting sugar content is an intricate trait controlled by multiple genetic and you will ecological things [a dozen, 13]. The brand new state-of-the-art hereditary buildings on the characteristic try consistent with the interest many nutrients mixed up in metabolic paths connecting starch malfunction and synthesis so you’re able to glucose creation and you will application [fourteen, 15]. Hereditary mapping studies have shown you to fry processing high quality and cutting sugars are on the thousands of short impression QTLs [sixteen,17,18,19,20]. Soltis-Kalina et al. identified one or two major QTLs to have processor colour for the chromosomes 1 and you will 6 inside an effective diploid mapping populace. More recently, Braun ainsi que al. claimed one or two QTLs for resistance to cooler-triggered sweetening to your chromosomes 4 and you will six from diploid potato. Most of these research has started performed for the diploid potato germplasm. Although not, then scientific studies are needed to most useful see the genetic buildings off these faculties at tetraploid top inside the populations produced from russet-skinned potato species.